Hello friends, in today's article, we see the MCQs of construction of Genomic DNA and cDNA library. In this, we see MCQs of Genomic, cDNA, etc.
so let's see one by one
Construction of Genomic DNA and cDNA Library MCQs:-
Construction of genomic and C DNA library:-
1. The first genomic libraries were cloned in _______________
a) Plasmid
b) Bacteria
c) Human
d) Plants
2. Genomic library construction is concerned with ___________
a) Gene isolation
b) Protein production
c) Antibiotics
d) Regeneration
3. Which DNA is restricted to making a genomic library?
a) Genomic
b) Plasmid
c) Phage
d) Plant
4. What is the approximate size of fragments given off by EcoR1?
a) 1 kb
b) 2 kb
c) 3 kb
d) 4 kb
5. A genomic library is a collection of _____________
a) Genes
b) Proteins
c) Vectors
d) Recombinants
6. If a gene is large, it may be cut more than once by an endonuclease.
a) True
b) False
7. The Clarke and Carbon formula relate the ____________ of including a DNA fragment in a random library.
a) Probability
b) Effects
c) Temperature change
d) Vector requirement
8. In the strategy devised by Maniatis, how many restriction enzymes were used?
a) 1
b) 2
c) 3
d) 4
(Construction of Genomic DNA and cDNA Library MCQs ask in CSIR NET Exam)
9. The restriction enzymes used by Maniatis had recognition sites of ____ nucleotides.
a) 1
b) 2
c) 3
d) 4
10. Which kind of packing is done for the fragmented genes?
a) In vivo
b) Population
c) Group
d) In vitro
11. HaeIII and AluI have ___________ recognition sites.
a) Different
b) Similar
c) Short
d) Unrecognizable
12. Linkers are required when using enzymes HaeIII and AluI.
a) True
b) False
13. The ‘Charon series’ belongs to series of ____________
a) Genes
b) Vectors
c) Hosts
d) Enzymes
14. Sau3AI is a _______ restriction endonuclease.
a) Single
b) Double
c) Hybrid
d) Weak
15. Lambda EMBL3 is a ____________
a) Gene
b) Phage
c) Phage Vector
d) Protein
16. How many types of DNA libraries are possible?
a) 1
b) 2
c) 3
d) 4
17. In the following type of libraries pick the odd one out.
a) A DNA library
b) B DNA library
c) cDNA library
d) Z DNA library
18. Which enzyme is involved in the synthesis of the DNA over an RNA template?
a) DNA polymerase
b) Reverse transcriptase
c) Klenow fragment
d) RNA polymerase
19. For the construction of the DNA library what is the range of genomic DNA that has to be cloned into the vector?
a) Less than 100 bases
b) 100 to 1000 bases
c) Less than 100 megabases
d) Less than 10 bases
(Construction of Genomic DNA and cD)NA Library MCQs ask in CSIR NET Exam)
20. Which of the following enzymes is responsible for the digestion of the RNA strand in the RNA-DNA hybrid?
a) RNase A
b) RNase H
c) DNase
d) Exonuclease
21. cDNA is synthesized from __________
a) Protein chain
b) tRNA
c) mRNA
d) DNA
22. During the storage of DNA fragments for making a library, larger fragments are having more chances of getting lost.
a) True
b) False
23. What is the characteristic of the DNA that is used for the construction of a library?
a) Naked DNA
b) Plasmid
c) Plasmid and naked DNA
d) Plasmid is preferred over naked DNA
24. What is the state of the phage cloning vector that is used for the construction of the library?
a) Packaged phage
b) Unpackaged phage
c) Packaged and unpackaged phage
d) Packaged and unpackaged phage are used but unpackaged phage is favored
25. Which enzyme is used to avoid ligation of separate DNA?
a) Kinase
b) Phosphatase
c) Endonuclease
d) Ligase
26. Partial digestion is preferred for the fragmentation of DNA.
a) True
b) False
27. The ordered steps for the construction of library involves __________
i) Vector preparation
ii) Amplification
iii) Ligation and introduction into the host
iv) Isolation of genomic DNA
v) Fragmentation of DNA
a) iv, v, i, iii, ii
b) v, iv, i, iii, ii
c) i, ii, iii, iv, v
d) ii, iii, iv, v, i
28. What is the significance of “c” in the cDNA library?
a) Copy DNA
b) Cut DNA
c) Cell DNA
d) Clone DNA
29. mRNA can be readily separated from lysed eukaryotic cells by adding magnetic beads. What is the sequence of this magnetic bead?
a) Oligo G
b) Oligo A
c) Oligo T
d) Oligo C
30. What is the significance of SI nuclease?
a) DNA synthesis
b) Cleavage of hooks
c) Degrades RNA
d) Annealing the primer
31. __________ is a collection of the total genomic DNA from a single organism.
a) Bacteriophage Lambda
b) Reporter Genes
c) Lambda Library
d) Genomic Library
32. The first DNA-based genome ever fully sequenced was achieved by two-time Nobel Prize winner, Frederick Sanger, in?
a) 1976
b) 1977
c) 1978
d) 1979
33. Genomic libraries are commonly used for sequencing applications
a) TRUE
b) FALSE
c) Can be true or false
d) Can not say
34. One major use of genomic libraries is hierarchical shotgun sequencing, which is also called?
a) top-down
b) map-based
c) clone-by-clone
d) All of the above
35. Cosmid vectors are plasmids that contain a small region of bacteriophage DNA called the cos sequence.
a) TRUE
b) FALSE
c) Can be true or false
d) Can not say
36. BACs stands for?
a) Bacteria artificial chromosomes
b) Bacteriophage artificial chromosomes
c) Bacterial artificial chromosomes
d) Bacterium artificial chromosomes
37. Libraries can broadly be classified into how many types?
a) 1
b) 2
c) 3
d) 4
38. Choose the correct statement for genomic libraries.
a) Genomic libraries include the representation of the whole genome of the organism
b) Sequences such as telomeres are also represented
c) Telomeres can be readily cloned
d) The larger the size of the insert of genomic DNA in recombinants, the more is the number of recombinants required to represent the genome in the library
39. Choose the incorrect statement for the preparation of genomic libraries.
a) The first step is the isolation of genomic DNA
b) Physical damage to the DNA should be avoided
c) If a nuclear DNA library is to be constructed, organelle DNA is to be removed
d) For the construction of the organelle library, organelle DNA is purified from the nuclear DNA
40. To avoid ligation of separate DNA fragments, which of the enzyme is used?
a) kinase
b) ligase
c) endonuclease
d) phosphatase
41. Genomic Library is normally made by
Alpha phage vector
Lambda phage vector
Beta phage vector
Gamma phage vector
42. A labeled DNA or RNA segment, which is used to find a specific sequence of nucleotides in a DNA molecule is named as
Vector
Probe
Sticky end
Destabilized DNA
43. Long probe is usually made by
Gene expression
Cloning
hybridization
PCR
44. Normally genomic library was made by
T4 phage
T3 phage
Lambda phage vectors
T6 phage
45. A piece of DNA and RNA used to Detect specific nucleic acid sequence by hybridization is called
A probe
Plasmid
Vector
Endonuclease
46. In Eukaryotic cells mature mRNA is already spliced, hence the cDNA produced have no
Introns
Exons
Ribosomes
Uracil
47. To express eukaryotic genes in prokaryotes the library used is
cDNA library
bDNA library
aDNA library
zDNA library
48. A technique that measures the degree of genetic similarity between pools of DNA sequence is called.
Annealing
Denaturation
Hybridization
Polymerization
49. The enzyme is used in the formation of cDNA from mRNA is
Helicase
Polymerase
Reverse transcriptase
Gyrase
50. cDNA is produced from
tRNA
mRNA
Protein Chain
DNA
(Construction of Genomic DNA and cDNA Library MCQs ask in CSIR NET Exam)
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Answer:-
1) A
2) A
3) A
4) D
5) D
6) A
7) A
8) B
9) D
10) D
11) A
12) A
13) B
14) A
15) C
16) B
17) C
18) B
19) C
20) B
21) C
22) A
23) C
24) B
25) B
26) A
27) A
28) A
29) C
30) B
31) D
32) B
33) A
34) D
35) A
36) C
37) B
38) A
39) C
40) D
41) B
42) B
43) B
44) C
45) A
46) A
47) A
48) C
49) C
50) B
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